GeneKam Biotechnology AG PCR檢測(cè)試劑盒-相關(guān)技術(shù)文章

更新時(shí)間：2024-07-19

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GeneKam Biotechnology AG PCR檢測(cè)試劑盒

Genekam Biotechnology AG公司簡(jiǎn)介

Genekam Biotechnology AG成立于1999年，從2004年至2015年已向全球70個(gè)國(guó)家提供PCR檢測(cè)試劑盒。

Genekam Biotechnology AG公司技術(shù)領(lǐng)域

Genekam檢測(cè)產(chǎn)品涵蓋流感病毒、中東呼吸綜合征病毒（MERS）、丙型肝炎病毒、登革熱、皰疹病毒、分枝桿菌、衣原體、支原體、沙門氏菌等食品病原體等。

Genekam致力于為大流行性病毒的預(yù)防、檢測(cè)和治療提供解決方案。

細(xì)胞培養(yǎng)基中2-3%的胎牛血清足以促進(jìn)人單核細(xì)胞培養(yǎng)物的發(fā)育和生長(zhǎng)

目的：通常的做法是，10-20%的胎牛血清（FCS）被用于細(xì)胞培養(yǎng)基中，用于人單核細(xì)胞以及其他細(xì)胞培養(yǎng)物的發(fā)育和維持。FCS是通過涉及動(dòng)物的痛苦過程產(chǎn)生的，是主要的成本因素之一。FCS數(shù)量的減少可以減少其不道德行為，并大大降低成本。

材料與方法：因此，將2-3%的FCS和10%的FCS用于開發(fā)不同的單核細(xì)胞培養(yǎng)物進(jìn)行比較，并測(cè)試了不同的參數(shù)，如形態(tài)學(xué)、抗體的產(chǎn)生、用微型柱和磁珠分離DNA/RNA細(xì)胞以及人類內(nèi)部對(duì)照的存在與實(shí)時(shí)PCR。

結(jié)果：結(jié)果發(fā)現(xiàn)，在上述參數(shù)中，2-3%的FCS和10%含FCS的培養(yǎng)基之間沒有差異。開發(fā)了一百多種這樣的細(xì)胞培養(yǎng)物，其中許多細(xì)胞培養(yǎng)物維持了 3 年以上。

結(jié)論：2-3%的FCS足以長(zhǎng)期開發(fā)和維持人類單核細(xì)胞培養(yǎng)物。

Genekam Biotechnology AG公司熱銷產(chǎn)品

產(chǎn)品名稱	貨號(hào)	規(guī)格
Parainfluenza virus 1,2,3 Conventional PCR kit	K062	100reactions
Human Interleukin-10real time PCR kit	FR415	100reactions
Magnetic Separation VEGFR2/CD39 Positive Cells (endothelial) (only magnetic beads)	STEM60	10 reactions
Polio Virus (human) Conventional PCR kit	K039	100reactions
Human Interleukin-13real time PCR kit	FR416	100reactions
Magnetic CD31 Positive Cells (endothelial) (only magnetic beads)	STEM61	10 reactions
PRRSV (porcine reproductive and respiratory syndrome virus) Conventional PCR kit	K042	100reactions
Human Interleukin-23-alfareal time PCR kit	FR422	100reactions
Magnetic bead antibody isolation	SB0142	2 reactions
Rotavirus Conventional PCR kit	K060	100reactions
Human Interleukin-5real time PCR kit	FR414	100reactions
Magnetic bead antibody isolation	SB0143	10 reactions
Rubella Virus (human) Conventional PCR kit	K838	100reactions
Human Interleukin-6real time PCR kit	FR421	100reactions
Endotoxin Removal Kit	SB0146A	1 ml
Sandfly fever sicilian virus Conventional PCR kit	K249	100reactions
Human Interleukin-8real time PCR kit	FR420	100reactions
Endotoxin Removal Kit	SB0146B	5 ml
Tick-borne encephalitis TBE (human) Conventional PCR kit	K034	100reactions
Human Papilloma virus real time PCR kit	FR039	100reactions
Endotoxin Removal Kit	SB0146C	10 ml
Universal Fluhunter 2: Universal PCR kit for Hemagglutinin gene (HA-gene) decoding and detection of influenza viruses + 10 genesequencings Conventional PCR kit	K336	100reactions
Human Papilloma Virus HPV-16 (high risk profile) real time PCR kit	FR041	100reactions
Erythrocyte removal kit (Sample)	SB0147	10 ml
Yellow fever YF Conventional PCR kit	K412	100reactions
Human Papilloma Virus HPV-18 (high risk profile) real time PCR kit	FR042	100reactions
Erythrocyte removal kit	SB0147S	100 ml
Arthropod-Borne Universal Flavivirus kit (It detects Dengue virus, West nile virus, JEV, TEBV, Yellow fever, St. Luis Encephalitis Virus and other flaviviruses of athropod like Mosquitoes an... Conventional PCR kit	K267	100reactions
Human Papilloma virus-HPV High Risk Genotyping (16,18,31,33) real time PCR kit	FR214	100reactions
Erythrocyte removal kit	SB0147M	200 ml
Fluhunter: Ready to use PCR kit for H3N2 (Iowa strain) Conventional PCR kit	K313	100reactions
Human Papilloma virus-HPV-33,51, 52 and 58real time PCR kit	FR370	100reactions
Human T Cell( CD4 Positive Cells ) magnetic beads isolation Kit (full kit)	SB0149	10 reactions
Fluhunter -Corona (Universal Kit) with 5 genesequencings Conventional PCR kit	K354	100reactions
Human Papilloma virus-HPV-35-53, 59 and 66real time PCR kit	FR371	100reactions
Human T Cell ( CD8 Positive Cells ) magnetic beads isolation Kit (full kit)	SB0150	10 reactions
Fluhunter -Corona (Universal Kit) with 25 genesequencings Conventional PCR kit	K355	100reactions
Human Papilloma virus-HPV-39real time PCR kit	FR373	100reactions
Human T Cell ( CD3 Positive Cells ) magnetic beads isolation Kit (full kit)	SB0151	10 reactions
Fluhunter-Corona Plus (Universal kit) with 5 genesequencings Conventional PCR kit	K356	100reactions
Human Papilloma virus-HPV-39, 45, 56 and 68real time PCR kit	FR369	100reactions
Human B Cell ( CD19 Positive Cells ) magnetic beads isolation Kit (full kit)	SB0152	10 reactions
Fluhunter-Corona Plus (Universal kit) with 50 genesequencings Conventional PCR kit	K358	100reactions
Human Papilloma virus-HPV-45real time PCR kit	FR374	100reactions
Human B Cell ( CD20 Positive Cells ) magnetic beads isolation Kit (full kit)	SB0153	10 reactions
Fluhunter Middle East SARS virus (Single Check):Ready to use PCR Kit to detect the middle east SARS virus Conventional PCR kit	K360	100reactions
Human Papilloma virus-HPV-52real time PCR kit	FR375	100reactions
Human B Cell ( CD27 Positive Cells ) magnetic beads isolation Kit (full kit)	SB0154	10 reactions
Fluhunter Middle East SARS virus (Double Check):Ready to use PCR Kit to detect the middle east SARS virus Conventional PCR kit	K361	100reactions
Human Papilloma virus-HPV-6 and 11real time PCR kit	FR372	100reactions