Cedarlane 淋巴細(xì)胞分離柱-相關(guān)技術(shù)文章

更新時(shí)間：2024-07-16

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Cedarlane 淋巴細(xì)胞分離柱

Cedarlane公司成立于加拿大，27年間，已經(jīng)逐步發(fā)展成為生命科學(xué)行業(yè)的一家跨國公司，在加拿大和美國擁有超過100名員工。

Cedarlane公司的研究領(lǐng)域。

公司為生命科學(xué)研究人員和臨床醫(yī)生提供無數(shù)的生物和生化產(chǎn)品，提供來自幾乎所有世jie上zui 知 ming 的國際制造商的產(chǎn)品。交貨及時(shí)，所有產(chǎn)品都在適當(dāng)?shù)乃铚囟认聝Υ妗⒔邮蘸瓦\(yùn)輸。員工與客戶和供應(yīng)商密切合作，提供個(gè)性化和全面的體驗(yàn)，以反映客戶至上的核心價(jià)值。通過提供全球超過 600 萬種試劑盒和試劑的門戶，客戶擁有貨運(yùn)整合的優(yōu)勢以及其中固有的便利性和成本節(jié)約;Cedarlane 是一站式商店。

主要分銷產(chǎn)品線包括：抗體、細(xì)胞培養(yǎng)基和過濾、原代細(xì)胞和細(xì)胞系、蛋白質(zhì)和肽、酶和抑制劑、成像和顯微鏡資源、檢測/ELISA試劑盒、精細(xì)化學(xué)品和生化、DNA/RNA分離和純化。

主要制造產(chǎn)品包括：單克隆和多克隆抗體、細(xì)胞分離培養(yǎng)基、組織分型補(bǔ)體和高度表征的神經(jīng)元細(xì)胞系。

Lympholyte®-H是一種密度梯度分離介質(zhì)，專門設(shè)計(jì)用于從人外周血和臍帶血中分離淋巴細(xì)胞和單核細(xì)胞。

注意：使用前充分搖晃混勻，靜止2-3min，待氣泡消失后試用。（室溫條件下使用分離液，分離液密度會隨溫度的變化而發(fā)生改變）

樣本制備可使用無血清培養(yǎng)基，減少樣本粘度，更利于分離。

操作步驟：

1.在含有抗凝血劑的試管中收集人血；

2.用等量培養(yǎng)基1：1稀釋血液；

3.根據(jù)不同的方法進(jìn)行分離：

①在離心管中加入3 mL Lympholyte®-H；

②使用移液管，小心地將6 mL稀釋的血液鋪在Lympholyte®-H上，在液面上盡可能少地混合（圖A）；

③由于Lympholyte®-H的密度比細(xì)胞懸液更大，因此將形成一個(gè)明顯的界面（圖C）。

方法B：

①在離心管中加入6 mL稀釋后的血液。將一個(gè)大的(23厘米）移液管放置到試管的底部（圖B）；

②慢慢地向移液管中加入Lympholyte®-H，重力使其在稀釋的血液下分層；

③繼續(xù)處理，直到3mL Lympholyte®-H在稀釋的血液下分層。由于Lympholyte®-H比細(xì)胞懸液密度更大，因此會形成明顯的界面（圖C）；

4.在室溫下，以800g離心20分鐘；

5.離心后，界面上將有一個(gè)明顯的淋巴細(xì)胞層（圖D）。使用移液管，小心地將細(xì)胞吸出，并轉(zhuǎn)移到一個(gè)新的離心管中；

6.用培養(yǎng)基稀釋轉(zhuǎn)移的細(xì)胞，以降低溶液的密度。800g離心10分鐘，沉淀淋巴細(xì)胞，然后棄用上清液；

Note：如果需要去除血小板，可在這一步進(jìn)行操作：

方法A

A.加入0.5 mL緩沖液和50 uL凝血酶，然后輕輕重懸細(xì)胞；

B.用緩沖液補(bǔ)足10mL，并充分混合；

C.非常緩慢地(100g）離心細(xì)胞3分鐘；血小板會聚集并沉淀在底部；

D. 取出上清液（包含細(xì)胞），放入另一個(gè)試管中，留下聚集的血小板。

方法B

A.800 g離心1分鐘，沉淀細(xì)胞；

B.倒出上清液，用緩沖液重懸細(xì)胞，重復(fù)2次；

C.此時(shí)會觀察到，當(dāng)血小板被移除時(shí)，產(chǎn)生的上清液會變得更加清晰。

7.在進(jìn)一步處理之前，在培養(yǎng)基中清洗淋巴細(xì)胞2-3次（在這個(gè)階段可以使用含有血清的培養(yǎng)基）。

Cedarlane 淋巴細(xì)胞分離柱-相關(guān)技術(shù)文章

以下是Cedarlane公司的熱銷產(chǎn)品

產(chǎn)品名稱	貨號	規(guī)格
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid	CL5020	500ml
Baby Rabbit Complement, Tissue Culture Grade - Frozen	CL3441-S	1ml
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid	CL5016	6x100mL
Low-Tox® Guinea Pig Complement, Lyophilized	CL4051	5x1ml
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid	CL5031	100ml
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid	CL5115	100ml
Low-Tox®-M Rabbit Complement, Lyophilized	CL3051	5x1ml
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid	CL5030	5x30ml
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid	CL5015	100ml
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid	CL5026	6x500ml
Anti-Mouse Asialo GM1, Ig Fraction (Polyclonal)(rabbit Ig)	CL8955	1 mL
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid	CL5120	500ml
Human Complement, Pooled, Frozen	CL6600-1	1 ml
Anti-Mouse/Human Mac-2 (Galectin-3), Purified (Clone M3/38) (rat IgG2a)	CL8942AP	100ug
Anti-Human Tamm Horsfall Purifed (Clone 10.32) (mouse IgG2b)	CL1032AP	250ug
Anti-Mouse Complement Component C3, FITC (Clone RmC11H9) (rat IgG2a)	CL7503F	100ug
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid	CL5110	5x30ml
Lympholyte®-1.1, sterile liquid	CL6155	500mL
Baby Rabbit Complement, Lyophilized	CL3441	10x1ml
Anti-Human Factor VIII:C HRPO (Polyclonal) (sheep IgG)	CL20035HP	0.2 mg
Anti-Human Factor VIII:C Aff.Pur.HRPO(Paired Abs)	CL20035K	4 plates
Low-Tox®-H Rabbit Complement, Lyophilized	CL3331	10x1mL
Chicken Gamma Globulin (purified antigen)	CLF802-1	50mg
Lympholyte®-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid	CL5041	100mL
Lympholyte®-Rabbit Cell Separation Media (Isolation of lymphocytes from Rabbit lymphoid tissue), sterile liquid	CL5050	5x30ml
Standard Rabbit Complement, Lyophilized	CL3111	25x1ml
Anti-Rat CD90 (Thy 1.1) Ascites (Clone OX-7) (mouse IgG1)	CL005A	0.5ml
Rabbit Complement MA, Lyophilized	CL3221	10x1ml
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid	CL5070	100mL
Anti-Human Fibrinogen Aff.Pur./HRPO (Paired Abs)	CL20067K	5 plates
Lympholyte®-1.1, sterile liquid	CL5095	500mL
O-sialoglycoprotein endopeptidase, Lyophilized	CLE100	1.2mg
Anti-Mouse Thrombocyte (platelet), Antiserum, (Polyclonal) (rabbit serum)	CLA31440	5mL
Anti-Human Tissue Plasminogen Activator, Affinity Purified (Polyclonal) (sheep IgG)	CL20115AP	0.5 mg
Anti-Human Factor IX Aff.Pur./HRPO (Paired Abs)	CL20041K	5 plates
Baby Rabbit Complement, Tissue Culture Grade, Frozen	CL3441-S100	100ml
Standard Guinea Pig Complement, Lyophilized	CL5000	5x5ml
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid	CL5010	5x30mL
Human Interleukin-17A	CL101-17-25UG	25ug
Anti-Mouse Collagen Type I, purified IgG	CL50151AP-1	0.1ml
Anti-Mouse CD25 , Purified (Clone PC61.5.3) (rat IgG1)	CL8925AP	250ug
Anti-Rat IL-10, Purified, (Polyclonal) (rabbit IgG)	CL9583AP	50ug
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid	CL5071	250 mL
Anti-Mouse PMN, Antiserum, (Polyclonal) (rabbit serum)	CLA31140	5mL
Anti-Rat Myeloid Lineage, FITC (Clone OX82) (mouse IgG1)	CL081F	100 ug
Anti-Rat Macrophage, Adsorbed Antiserum, (Polyclonal) (rabbit serum)	CLAD51240	2mL
Baby Rabbit Complement- Tissue Culture Grade, Frozen	CL3441-S50	50 ml
Recombinant Human Gastrokine 3	CLPRO2033-2	10ug
Anti-Rat CD45RA/B FITC (Clone OX-33) (mouse IgG1)	CL033F	100ug
Mini Super PAP PEN (tip 1/10" diameter, 3 mL)	MU22	EA
Anti-Rat CD11b/c Purified (Clone OX-42) (mouse IgG2a)	CL042AP	250ug
Anti-Human Factor H (FITC) (Clone: OX-24) (mouse IgG1)	CL7672F	100 ug
Anti-Mouse I-Ad, Ascites (Clone 34-5-3S) (mouse IgG2a)	CL8713A	0.5 mL
Anti-Human Antithrombin III Affinity Purified (Polyclonal) (sheep IgG)	CL20015AP	0.5mg
Cedarlane/Anti-Rat CD45, 10 nm Gold Conjugate,OD3 (Clone OX-1) (mouse lgG1)	CL009G	0.5 mL
Cedarlane/Rat IL-2ELISA kit(precoated)	CL00113K-96	96T